The spectrophotometric determination of the oxygen saturation of blood.
نویسندگان
چکیده
ORDY AND DRABKIN (1) have described a simplified spectrophotometric procedure for the determination of oxygen saturation of whole hemolyzed blood. The described method of sampling by use of tonometers, although technically precise, is somewhat inconvenient for routine clinical use. In a note appended to the article, Marsh described a simpler modification. He collects the samples in oiled, heparinized syringes and adds saponin solution directly by means of a syringe and three-way metal stopcock. A simple, open, 1 cm. absorption cell with a 9 mm. lucite plug, giving a light path of 1 mm., was substituted for the more complicated anaerobic cell of 0-ordy and Drabkin. We desired a method that would permit rapid handling of blood samples removed during cardiac catheterization or open heart surgery and that would make available aliquots for duplicate analyses or other analytical procedures such as hemoglobin or hematocrit estimations. We modified the technic described by Marsh in that a 1 ml. portion of the sample is transferred anaerobically to a 1 ml. tuberculin syringe by means of a three-way stopcock. Hemolysing and reducing solutions may then be added through the same stopcock and mixed with the sample before transfer to an absorption cell.
منابع مشابه
Spectrophotometric determination of the oxygen saturation of whole blood.
A new approach to the spectrophotometric determination of blood oxygen saturation has been made which is based on the absorption maximum of oxyhemoglobin and the isosbestic point of oxyhemoglobin and hemoglobin. The precision of this new method is comparable to that of the manometric procedure in which the Van Slyke-Neil1 apparatus is employed; there is also good correlation between results obt...
متن کاملA modification of the spectrophotometric determination of carbon monoxide in blood enabling direct determination of per cent hemoglobin saturation.
BECAUSE OF VARIATION in hemoglobin concentration in normal bloods with age and sex (1), the per cent carbon monoxide saturation of hemoglobin represents a more accurate toxicity index than the volume per cent of carbon monoxide. In 2 of the most common methods of carbon monoxide determination in blood, the gasometric method of Van Slyke (2, 3, 4) and the spectrophotometric method of Harper (5),...
متن کاملSpectrophotometric studies. XVI. Determination of the oxygen saturation of blood by a simplified technique, applicable to standard equipment.
The metabolism, as measured by oxygen consumption, of organs in situ is being increasingly investigated by the vascular catheterization technique (see Drabkin (3) for pertinent literature). The oxygen consumption is derived from the arteriovenous difference in the oxygen saturation or percentage of oxyhemoglobin (4) of blood entering and leaving the tissue. Such work has pointed to the need of ...
متن کاملA simple spectrophotometric method for the determination of blood sugar by phenylhydrazine Hydrochlorlde
متن کامل
Spectrophotometric determination of blood oxygen.
Gasometric methods for estimating blood oxygen are time-consuming. This consideration often limits the number of observations which can be made in the course of experiments requiring oxygen determinations. In many cases it is an arteriovenous difference which is particularly desired, as in the measurement of cardiac output by the Fick principle, or the estimation of oxygen consumption by human ...
متن کاملبررسی و مقایسه تاثیر دیازپام وریدی با میدازولام وریدی به عنوان پیش داروی بیهوشی بر چگونگی اشباع اکسیژن خون شریانی قبل از شروع عمل جراحی در بیماران ریسک یک تحت بیهوشی عمومی در اطاق عمل یکی از ...
This research, which is semi- experimental is focused on the influence and comparison intravenous diazepam with intravenous midazolam as premedication in anesthesia on the saturation arterial blood oxygen before surgical operation, "pan patients who are in risk one under general anesthesia in the operating rooms of the hospitals affiliated to Iran University of Medical Sciences and Heal...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 5 شماره
صفحات -
تاریخ انتشار 1959